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アイテムタイプ	紀要論文
タイトル	Smooth sectioning of biological samples by FIB-TOF-SIMS
著者	著者名 Goto, Tomoki
	著者名 Takeuchi, Hyoga
	著者名 Shu, Mizuki
	著者名 Saito, Reiko
	著者名 Matsushima, Kazuya
	著者名 Saito, Makoto
	著者名 Hanada, Sanshiro
	著者名 Kojima, Nobuhiko
	著者名 Morita, Masato
	著者名 Sakamoto, Tesuo
言語	eng
ISSN	02860201
DOI	https://doi.org/10.15002/00025851
出版者	法政大学イオンビーム工学研究所
雑誌名	PROCEEDINGS OF THE 40th SYMPOSIUM ON MATERIALS SCIENCE AND ENGINEERING RESEARCH CENTER OF ION BEAM TECHNOLOGY HOSEI UNIVERSITY (December 8, 2021)
巻	40
開始ページ	10
終了ページ	15
発行年	2022-02-18
著者版フラグ	Version of Record
キーワード	Spheroids
	FIB-TOF-SIMS
抄録	Spheroids, which are three-dimensionally cultured cells that resemble actual living organisms, have been attracting attention. FIB-TOF-SIMS (Focused Ion Beam Time-of-Flight Mass Spectrometry) is capable of simultaneous mass imaging of multiple elements without the need for labeling. FIB-TOF-SIMS is expected to process the spheroid and image the cross-sectional components. However, FIB processing of spheroids larger than 100μm often results in uneven cross sections due to the so-called curtain effect. The unevenness of the cross-section affects the sputtering and hinders component imaging. In this experiment, we considered the processing of spheroids by FIB from multiple directions to suppress the curtain effect. The curtain effect was evaluated by comparing the processing from one direction and from multiple directions.
資源タイプ	Article
インデックス	資料タイプ別 ＞ 紀要 ＞ PROCEEDINGS OF SYMPOSIUM ON MATERIALS SCIENCE AND ENGINEERING RESEARCH CENTER OF ION BEAM TECHNOLOGY HOSEI UNIVERSITY
	216 イオンビーム工学研究所 ＞ 紀要 ＞ PROCEEDINGS OF SYMPOSIUM ON MATERIALS SCIENCE AND ENGINEERING RESEARCH CENTER OF ION BEAM TECHNOLOGY HOSEI UNIVERSITY ＞ 40

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