紀要論文

大腸菌増殖に不要な二成分制御系センサーキナーゼ

ALL SENSOR HISTIDINE KINASES ARE NOT ESSENTIAL FOR E SCHERICHIA COLI GROWTH

jpn

24368083

https://doi.org/10.15002/00030794

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学研究科編

65

1

2

2024-03-24

Version of Record

Two-component system (TCS) is a conserved stress response systems among prokaryotes. TCS consists of sensor kinase (SK) and response regulator (RR) and mainly regulates genome expression pattern. The all TCS gene-defective Escherichia coli strains, all 30 SK gene-defective ΔSK and all 34 RR gene-defective ΔRR strain, are isolated. Phenotype microarray showed that ΔSK was able to grow under several stress conditions but ΔRR was not. Metabolome showed that ΔSK significantly increases intracellular level of amino acids, such as branched chain amino acids (BCAA), in comparison to the parent. I performed transcriptome and proteome measured the promoter activity of BCAA metabolic gene in ΔSK, resulting that the promoter activity and mRNA level of all BCAA genes were similar to the parent strain whereas all proteins level increased in ΔSK. To identify the gene involved in the amino acid increase of ΔSK, transposon directed insertion site sequencing (TraDIS) was done using λ phage including mini-Tn10. As a result, more than 400 genes were detected as no essential genes for ΔSK growth.

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