紀要論文

大腸菌定常期細胞の増殖開始における不可逆性

IRREVERSIBLE DIFFERENTIATION OF ESHERICHIA COLI STATIONARY PHASE CELL DURING RE-GROWTH.

jpn

24368083

https://doi.org/10.15002/00030793

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学研究科編

65

1

2

2024-03-24

Version of Record

The growth of E. coli consists of three phases: lag phase, log phase, and stationary phase. When stationary phase cell is transferred to a fresh medium or broth, it prepares to start to grow at lag phase. To start re-growth, an intracellular proteins profile has to be optimized. ATP-dependent protease Clp, which is known as ClpXP and ClpAP in E. coli, organizes intracellular functional proteins. To understand cellular localization of Clp protease in living E. coli cells, ClpP fused to a green fluorescent protein (GFP) was observed with microscope. Clp diffused in logarithmic growth phase cells but localized at either of cell pole of stationary phase cells. And I found that Clp diffused in areas that whole cell in logarithmic growth phase cells. Single cell growth was observed by timelapse imaging, resulting that Clp maintained the localization in only a stationary phase cell whereas no Clp was found in daughter cells. Taken together, Clp protease irreversibly localizes in stationary phase cell, which could contribute to produce new daughter cell.

Article