紀要論文

江戸東京野菜「東京ウド」に発生した新種植物ウイルスの特性解明とゲノム配列の決定

CHARACTERIZATION AND GENOME SEQUENCE OF A NEW PLANT VIRUS PRESUMED TO BE CLASSIFIED IN THE GENUS PELARSPOVIRUS ISOLATED FROM ARALIA CORDATA, “TOKYO UDO”, AS ONE OF “EDO-TOKYO VEGETABLE”

jpn

24368083

https://doi.org/10.15002/00026456

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学研究科編

64

1

3

2023-03-24

Version of Record

A virus disease showing mosaic symptoms was observed on Aralia cordata in a production field in Tokyo. Mechanical inoculation with crude sup to test plants showed necrotic or chlorotic spots on Chenopodium quinoa inoculated leaves, followed by establishment of isolates in each. Both isolates were purified by sucrose density gradient centrifugation, and spherical particles of about 35 nm in diameter were observed under a transmission electron microscope. In addition, a specific protein of about 37 kDa and RNA of 4~5 kb were observed in both. RT-PCR with a commercially available PCR Primer for Carmovirus-Pelarspovirus (Agdia) amplified the DNA fragment, which was used as the basis for deciphering a portion of the genome by primer walking. The sequences of both isolates were generally identical, and BLAST searches showed about 60% homology with viruses of the genus Pelarspovirus, which is in agreement with the criteria for species identification of the genus (both of homologies for amino acid sequence of RNA dependent RNA polymerase and coat protein <75%, respectively), indicating that this virus is likely to be a new species.

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