紀要論文

光架橋法と蛍光観察による大腸菌べん毛モーター固定子ユニットのダイナミクスの解明

DYNAMICS OF THE STATOR UNITS IN Escherichia coli FLAGELLAR MOTOR REVEALED BY PHOTO-CROSSLINKING AND FLUORESCENCE OBSERVATION

jpn

24368083

https://doi.org/10.15002/00025418

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学研究科編

63

1

2

2022-03-24

Version of Record

The bacterial flagellar motor consists of a rotor and ～10 stator complexes, MotA/MotB. Its rotational torque is generated by interactions between the cytoplasmic region of stator protein MotA and the C-terminal region of rotor protein FliG. By changing the conformation of periplasmic domains of MotB to anchor to the peptidoglycan layer, each stator complex opens its proton channels and works as a stator. In this study, we observed the dynamics of eGFP-fused MotB by TIRF microscopy, after jamming the stator-rotor interactions by in vivo photo-crosslinking. The UV intensity was adjusted to form one or a few cross-links between a rotor and stator units. From the results of fluorescence observation, it was found that the stator units dissociated from the motor within 30 s to 1 min after jamming the motor rotation. Interestingly, a short time after the rotation was jammed, some motors re-started to rotate. These results suggest that the stator unit, which was cross-linked to the rotor, was inactivated and no longer bound to the peptidoglycan layer, allowing it to rotate with the rotor.

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