紀要論文

Vibrio choleraeセリン走性に関わる可溶性受容体と膜貫通型トランスデューサーをコードする遺伝子の発現調節

TRANSCRIPTIONAL REGULATION OF THE VIBRIO CHOLERAE GENES ENCODING A SOLUBLE RECEPTOR AND A TRANSMEMBERANE TRANSDUCER FOR SERINE TAXIS

jpn

24368083

https://doi.org/10.15002/00025415

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学研究科編

63

1

2

2022-03-24

Version of Record

Vibrio cholerae, the causative agent of cholerae disease, migrates by rotating a polar flagellum towards various amino acids. Multiple amino acids including L-serine are sensed by the major chemoreceptors Mlp24 and Mlp37, both of which are supposed to play important roles in infection．However, a double mutant lacking the mlp24 and mlp37 genes still shows weak but significant responses to L-serine, suggesting that the bacterium has other amino acid receptor(s). Further studies identified an amino acid sensing system of V. cholerae consisting of a transmembrane transducer Mlp3 and a periplasmic soluble receptor named SatA. In this study, I found that the satA promoter is activated under nutrient-poor conditions, whereas the mlp3 promoter is activated under nutrient-rich conditions. Cells cultured under either conditions did not show significant serine responses. Other factors (e.g., other carbon sources, temperature, oxygen availability) may have to be tuned for the SatA-Mlp3 sensing system.

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