紀要論文

環境変化に応じた大腸菌異物排出トランスポーターの転写誘導と三量体形成

TRANSCRIPTIONAL INDUCTION AND TRIMER FORMATION OF THE ESCHERICHIA COLI EFFLUX TRANSPORTERS IN RESPONSE TO ENVIRONMENTAL STIMULI

jpn

24368083

https://doi.org/10.15002/00025414

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学研究科編

63

1

2

2022-03-24

Version of Record

The resistance-nodulation-cell division (RND) tripartite complexes are the major xenobiotic efflux systems in Escherichia coli. Each consists of an inner membrane transporter, a membrane fusion protein, and the common outer membrane channel TolC. Among five such systems, only the AcrAB-TolC complex exists constitutively, and some other transporter genes, including acrD and mdtBC, is induced by indole via the two-component system BaeSR. This laboratory found that deletion of tolC induces AcrD, but further deletion of the tryptophanase gene tnaA abolishes this induction. Here I constructed a series of chimeric BaeS with other sensor kinases. The results suggest that BaeS senses the increase in the intracellular indole concentration. I also performed single molecule imaging of other transporters MdtBC, encoded in the same operon as baeSR, and found that they form heterodimers preferentially in the presence of substrates.

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