紀要論文

強光ストレス下または高温ストレス下でおこる光化学系II反応中心タンパク質の損傷に関する研究

STUDY ON DAMAGE TO REACTION CENTER PROTEINS OF PHOTOSYSTEM II UNDER STRONG LIGHT STRESS OR HIGH TEMPERATURE STRESS

jpn

24368083

https://doi.org/10.15002/00025411

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学研究科編

63

1

2

2022-03-24

Version of Record

Strong light stress or moderate heat stress damages the D1 protein of photosystem II (PSII) reaction center. Although the mechanisms of photodamage to the D1 protein have been intensively studied using isolated PSII preparations, the mechanisms of photodamage and heat damage to the D1 protein in vivo are still unknown. In this study, damage to the D1 protein under heat stress was compared with that under strong light stress using unphosphorylated or phosphorylated spinach thylakoid membranes in which almost all the D1 protein was either unphosphorylated or phosphorylated. Fragments of the D1 protein derived from cleavage in the DE loop (N22-23 kDa and C9 kDa fragments) were generated both upon treatment with strong light and moderate heat. These results suggest that phosphorylation of the D1 protein does not affect the overall pattern of damage to the D1 protein.

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