紀要論文

鞭毛軸糸の維持機構に異常を持つ新規クラミドモナス突然変異株lpp2の解析

Analyses of a novel Chlamydomonas mutant, lpp2, that has defects in maintaining structural integrity of flagellar axonemes

jpn

21879923

https://doi.org/10.15002/00022104

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学・工学研究科編

60

1

2

2019-03-31

Version of Record

The axoneme, the inner structure of cilia and flagella, must be flexible enough to allow sliding between the microtubules during flagellar beating, and rigid enough to maintain itself protruded from the cell body under the tension of membrane. However, little is known about how the axoneme equips these apparently inconsistent properties. In this study, I isolated and analyzed a novel Chlamydomonas mutant, named lpp2, whose flagellar axonemes spontaneously deform. The lpp2 flagella once protrude from cell bodies and beat normally, but axonemes in a part of the flagella were circularized within the flagellar membrane, after IFT particles were abnormally accumulated in the flagella. These observations suggest that this mutant has defects in maintaining structural integrity of the axoneme. Genetic mapping of the mutation and DNA sequencing of four genes in the mapped area revealed that the lpp2 has a mutation in MOT30, a gene common to organisms having motile cilia.

Article

AA12677220