紀要論文

心臓組織片と分散培養筋細胞の拍動同期過程

SYNCHRONIZATION PROCESS OF BEATING BETWEEN CARDIAC TISSUE PIECES AND DISPERSED CULTURE OF CARDIOMYOCYTES

jpn

21879923

https://doi.org/10.15002/00021628

法政大学大学院理工学研究科

法政大学大学院紀要. 理工学・工学研究科編

59

1

2

2018-03-31

Version of Record

ヘッダー部分に誤記 ; (誤) 法政大学大学院理工学・工学研究科紀要 (正) 法政大学大学院紀要. 理工学・工学研究科編

Heart is a systematic organ such as sinoatrial nod working as pacemaker. To validate the robustness of network for beating, cardiac tissue pieces of chick embryos (E7-9) were contacted dispersed culture of cardiomyocytes derived from chick embryos (E7) on micro electrode arrays, and I measured extracellular potential by multi electrode array (MEA) system. In analyzing beat synchronization, I calculated the inter-spike interval (ISI) as beating intervals. For verification of beat synchronization, I took difference between the beat time of the tissue pieces and the beat time of the cell cluster. In the time to beat synchronization, tissue pieces of ventricle were earlier compared with tissue pieces of atrium, but there were no significance. Furthermore, the pacemaker of beat at synchronization didn’t have key difference in atrium and ventricle. From the results, it was suggested that ISI of the pacemaker tended to stable and short than followed the other before the time of synchronization regardless of tissue and cell cluster. However, the pacemaker of beating wasn’t constantly same and interchanged during measurement the extracellular potential. Thus, it can't be stated unconditionally that superiority or inferiority in robustness of beating determine about cardiac slice pieces and cell cluster.

Article

AA12677220