紀要論文

ビフィズス菌と乳酸菌の分泌化合物による 大腸菌 uhpT 遺伝子の発現誘導機構

jpn

21879923

https://doi.org/10.15002/00014372

法政大学大学院理工学・工学研究科

58

1

2

2017-03-31

Version of Record

ヘッダー部分に誤記 ; (誤) 法政大学大学院理工学・工学研究科紀要 (正) 法政大学大学院紀要. 理工学・工学研究科編

Variety of microorganisms inhabit in the human intestine. More than 1,000 species of bacteria exist in the intestinal tract. Among these bacteria, each bacterial cell communicates to others, forming community to survive in the human intestine. Recently we found that supernatant of Bifidobacterium longum culture specifically induces the expression of Escherichia coli uhpT gene, encoding hexose-6-phosphate transporter. I analyzed the induction of E. coli uhpT gene by other bacteria, indicating that the uhpT promoter was induced by supernatant from culture of 7 species of Bifidobacterium, including B. longum and Lactobacillus rhmnosus but not E. coli. Heat-treatment and dialysis experiments showed signal(s) in supernatant of B. longum was heat-stable and small molecule. The uhpT promoter is known to be activated by UhpABC and Crp regulatory systems. I found that the uhpT promoter was not induced by supernatant of B. longum in all of the uhpA, uhpB, uhpC, and crp deleted mutants. Taken all results together, I suggest that Bifidobacterium and Lactobacillus secrete signal small molecule(s), which specifically induce the hexose-6-phosphate transporter gene of E. coli. Key Words : Eschcerichia coli, Bifidobacterium, Lactobacillus, uhpT, cell-cell communication

Article

AA12677220