紀要論文

大腸菌核様体タンパク質　H-NSとDNAの複合体の解析

jpn

21879923

https://doi.org/10.15002/00014371

法政大学大学院理工学・工学研究科

58

1

2

2017-03-31

Version of Record

ヘッダー部分に誤記 ; (誤) 法政大学大学院理工学・工学研究科紀要 (正) 法政大学大学院紀要. 理工学・工学研究科編

The bacterial chromosome is associated with a number of nucleoide proteins, most of which are a bifunctional DNA-binding protein playing roles in both DNA compaction and gene regulation. The E. coli genome is associated with at least six DNA-binding proteins, Dps, Fis, H-NS, HU, IHF, and StpA. During the transition of E. coli cells from logarithimic phase to stationary phase, the morphology of the nucleoid changes from a fibrous state to a rod form with change in the cellular composition of associated nucleoid proteins. Among six nucleoide proteins of E. coli, the level of HU and H-NS are almost constant while the level of others dramatically changed in the cell. Reporter analysis showed that hns promoter dramatically increased at stationary phase, suggested that H-NS play an important role to form nucleoide in logarithmic phase cell. First, I examined to observe single complex of liner DNA with H-NS with atomic force microscope. In the absence of protein, the liner DNA in the length of 1 kbp was found to be 300 nm microfiber. In the presence of H-NS, the DNA-protein complexes formed thick filaments in the half length of intact DNA. These observations indicate that H-NS were capable of forming shorted DNA filament. Key Words : Escherichia coli , nucleoid associated protein , H-NS

Article

AA12677220