紀要論文

大腸菌二成分制御系因子の包括的発現解析

COMPREHENSIVE ANALYSIS OF EXPRESSION OF TWO-COMPONENT SYSTEM FACTORS IN ESCHERICHIA COLI.

jpn

21879923

https://doi.org/10.15002/00010398

法政大学大学院理工学・工学研究科

55

1

2

2014-03-24

Version of Record

Single-cell bacteria, like Escherichia coli, adapts to various diverse change of environments for survival in nature. E. coli is exposed to acidic conditions in stomach and then to anaerobic conditions within intestine and survives by changes of the expression pattern of its genome. The selective expression of genes in a total of about 4,500 genes on its genome takes place by controlling the promoter recognition properties of RNA polymerase modulated by transcription factors. Response regulators (RR) are widely distributed among bacteria and belong to bacterial signal transduction systems, two-component system (TCS). In this system, a sensor kinase protein senses environmental signal, phosphorylate itself, and then transfer the phosphate to a RR to regulate gene expression. E. coli K-12 genome sequence predicted that a total of 30 sensor kinases and a total of 32 response regulators. To gain insight of the expression profile for TCS factors under several conditions, the strength of whole transcription factor gene-associated promoters was examined in vivo by using gfp reporter assay. First, we constructed a collection of reporter plasmid of E. coli promoters for transcription factors. In this study, we reported the expression profile for TCS factors at logarithmic and stationary phases under both aerobic and anaerobic conditions.

Article

AA12677220